Compare Regions of Interest
Jen Garbarino
16 steps
43 seconds
Atlasxomics
Latch
The compare tab of AtlasXPlots allows for the comparison of categorical observations in the h5 viewer.
1
Click on the Compare tab to get started
Setting up the Compare Workflow
2
Select an observation for comparison
If you just created the observation, you may need to open and close this selection dropdown once or twice to have it update with your latest observation
3
Click the category you would like for group A
4
Click the category you would like for group B
5
If needed, you can click the dropdown to see the full list of barcode identities for each group
6
Click "Confirm Cell Selections" when ready to perform the comparison
7
Add an output directory name for the comparison. Use a descriptive name to remember the comparison groups in the future
8
Choose the reference genome for the dataset
9
Click "Launch Workflow" to begin the workflow
10
The workflow will begin running the analysis.
These calculations will take time depending on the number of datapoints in your comparison. You can close the plots and it will not stop the workflow from running
11
Once competed, the workflow will say succeeded.
Viewing Comparison Results
12
Once finished you can load in the data by clicking "Fetch Workflow Results"
13
If using a previously created comparison from this session, click load in previous compare Workflow data to point to the correct files for the comparison of interest.
14
Comparisons are saved in the compare_outs folder in the data tab of your workspace.
15
Once the data is retrieved, you can visualize the differential gene and motif data through volcano plots and ranked plots
16
The full data tables and bigwig files for the comparison are also able to be visualized here.
Compare Regions of Interest
Jen Garbarino
16 steps
43 seconds
Atlasxomics
Latch
The compare tab of AtlasXPlots allows for the comparison of categorical observations in the h5 viewer.
1
Click on the Compare tab to get started
Setting up the Compare Workflow
2
Select an observation for comparison
If you just created the observation, you may need to open and close this selection dropdown once or twice to have it update with your latest observation
3
Click the category you would like for group A
4
Click the category you would like for group B
5
If needed, you can click the dropdown to see the full list of barcode identities for each group
6
Click "Confirm Cell Selections" when ready to perform the comparison
7
Add an output directory name for the comparison. Use a descriptive name to remember the comparison groups in the future
8
Choose the reference genome for the dataset
9
Click "Launch Workflow" to begin the workflow
10
The workflow will begin running the analysis.
These calculations will take time depending on the number of datapoints in your comparison. You can close the plots and it will not stop the workflow from running
11
Once competed, the workflow will say succeeded.
Viewing Comparison Results
12
Once finished you can load in the data by clicking "Fetch Workflow Results"
13
If using a previously created comparison from this session, click load in previous compare Workflow data to point to the correct files for the comparison of interest.
14
Comparisons are saved in the compare_outs folder in the data tab of your workspace.
15
Once the data is retrieved, you can visualize the differential gene and motif data through volcano plots and ranked plots
16
The full data tables and bigwig files for the comparison are also able to be visualized here.
