External: Germplasm v1.0

Germplasm is defined as any biological unit capable of reproduction via any method: sexual, asexual, natural and artificial. Germplasm can be defined as an individual, clone, family or population. Germplasm may exist in situ, vivo or vitro; reside in inventory; or no longer exist (historical record) or yet to exist (planned crosses). Germplasm is created via a breeding method known or unknown (ie. crossing, cloning, open pollination, double haploid, etc).

• Please note that germplasm and ontology must be uploaded into DeltaBreed before you attempt to upload experiments, observations or sample submissions.

Skills needed

This training module assumes that you know how to login, use practice servers, create and manage programs. If you need to review previous training materials, head back to the main training page and check out DeltaBreed servers, System Administration, and Program Administration.

Learning targets

These training materials will walk you through everything you want to know about germplasm in DeltaBreed. At the end of this module you will be able to:

• Download the germplasm template and add required germplasm information.

• Import germplasm, search germplasm, and use the pedigree viewer.

• Download germplasm lists and use the GID and/or entry numbers to append pedigree information.

• Create a list of germplasm that already exists in DeltaBreed.

Germplasm template

Import germplasm, search germplasm and use pedigree viewer

Add pedigree connections using entry numbers

Create a germplasm list and append pedigree connections using GIDs

Learning Link!

Review the following examples of how to add pedigree connections with entry numbers or GIDs.

1. Create a germplasm list and use entry numbers to establish pedigree connections.

1. Download the germplasm template from "import data" in DeltaBreed.

2. Create a new list of germplasm that includes a bi-parental cross from two cultivares and then single seed descent for 8 generations. For animal species, all individuals should be assigned biparental cross as a breeding method. You will need to assign entry numbers to each entry in the list. Then, use the entry numbers to reference germplasm within this new list to create pedigree connections.

3. Save and upload the new file into DeltaBreed. Go to the "Germplasm" view screen and to the pedigree viewer to check your pedigree connections to one of your single seed decent germplasm.

2. Add pedigree connections to germplasm that is already in DeltaBreed using GIDs.

1. Download the germplasm template from "import data" in DeltaBreed.

2. Create a list of germplasm that includes a bi-parental cross from two cultivares and then single seed descent for 8 generations. For animal species, all individuals should be assigned biparental cross as a breeding method. Keep in mind that you need to include name, breeding method, and source in order to successfully upload the file. Keep pedigree connections clear for the first upload.

3. Once you have successfully uploaded your germplasm, download the list you just created in DeltaBreed, add pedigree connections for your germplasm using the newly system assigned GID numbers within that file and re-import that data as a second list.

4. Once you have successfully uploaded your second list, head to the "germplasm" view screen in DeltaBreed and to the pedigree viewer to check your pedigree connections to one of your single seed decent germplasm.

Where to next?

Head back to the main DeltaBreed training page by closing this browser tab

or,

Head to the next training module, Ontology.